منابع مشابه
Optimal Financial Nä ı veté
When agents first become active investors in financial markets, they are relatively inexperienced. Much of the literature focuses on the incentives of presumably sophisticated informed agents to produce information, and not on the na¨ıve agents. However , unsophisticated agents are important aspects of financial markets and worth analyzing further. In this paper, we provide a theoretical perspe...
متن کاملAsymmetric protonation of EmrE.
The small multidrug resistance transporter EmrE is a homodimer that uses energy provided by the proton motive force to drive the efflux of drug substrates. The pKa values of its "active-site" residues--glutamate 14 (Glu14) from each subunit--must be poised around physiological pH values to efficiently couple proton import to drug export in vivo. To assess the protonation of EmrE, pH titrations ...
متن کاملParallel topology of genetically fused EmrE homodimers.
EmrE is a small H+-coupled multidrug transporter in Escherichia coli. Claims have been made for an antiparallel topology of this homodimeric protein. However, our own biochemical studies performed with detergent-solubilized purified protein support a parallel topology of the protomers. We developed an alternative approach to constrain the relative topology of the protomers within the dimer so t...
متن کاملNew free-exchange model of EmrE transport.
EmrE is a small multidrug resistance transporter found in Escherichia coli that confers resistance to toxic polyaromatic cations due to its proton-coupled antiport of these substrates. Here we show that EmrE breaks the rules generally deemed essential for coupled antiport. NMR spectra reveal that EmrE can simultaneously bind and cotransport proton and drug. The functional consequence of this fi...
متن کاملMAS solid-state NMR studies on the multidrug transporter EmrE.
We study the uniformly 13C,15N isotopically enriched Escherichia coli multidrug resistance transporter EmrE using MAS solid-state NMR. Solid-state NMR can provide complementary structural information as the method allows studying membrane proteins in their native environment as no detergent is required for reconstitution. We compare the spectra obtained from wildtype EmrE to those obtained from...
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ژورنال
عنوان ژورنال: Journal of Turkish Studies
سال: 2010
ISSN: 1308-2140
DOI: 10.7827/turkishstudies.1414